《实用免疫细胞与核酸》 九、常用酸碱技术参数

1．

溶质分子式分子量mol/Lg/L重量（%）比重配制mol/L溶液的加入量（ml/L）冰乙酸CH3COOH60.0517.40104599.51.05057.5乙酸60.056.27376361.045159.5甲酸HCOOH46.0223.401080901.20042.7盐酸HCl36.5011.60424361.18086.22.90105101.050344.8硝酸HNO363.0215.991008711.42062.514.90938671.40067.113.30837611.37075.2高氯酸HclO3100.5011.651172701.67085.89.20923601.540108.7磷酸H2PO480.0018.101445851.70055.2硫酸H2P｀O498.1018.001776961.84055.6氢氧化铵NH4OH35.0014.80251280.89867.6氢氧化钾KOH56.1013.50757501.52074.11.94109101.090515.5氢氧化钠NaOH40.0019.10763501.53052.42.75111101.110363.4

2．各种浓度的酸碱贮存液的近似pH值

溶质1Na0.1Na0.01Na0.001Na乙酸0.402.903.403.90盐酸0.101.072.023.01硫酸0.301.202.10柠檬酸2.102.60氢氧化铵11.8011.3010.8010.30氢氧化钠14.0513.0712.1211.13碳酸氢钠8.40碳酸钠11.5011.00

J a.N为光量浓度[1N≈（1mol/L）×离子价数]。